The walk-out comes as violent and sometimes deadly protests continue amid a political and economic crisis that has led to shortages of basic goods and soaring inflation.
"We are calling out the entire people, all groups in society, for a 48-hour strike" Wednesday and Thursday, lawmaker Simon Calzadilla said.
Calzadilla said that the strike would be capped on Friday with a march demanding that Maduro officially scrap his Constituent Assembly vote scheduled for July 30.
Earlier on Saturday, police on motorcycles fired tear gas to break up an opposition march on the Supreme Court to press demands that elected socialist Maduro leave office.
That rally was also meant as a show of support for a slate of 33 magistrates -- a so-called shadow supreme court -- whose names were put forward Friday by the opposition to replace Venezuela's current high court, which is closely allied with Maduro and frequently rules in his favor.
Emboldened by a nationwide strike on Thursday that paralyzed parts of the capital Caracas and other Venezuelan cities, opposition leaders held a mock swearing-in ceremony Friday for the shadow court's new "judges."
Many of the actual court's justices were hastily appointed shortly before Maduro's ruling party lost its majority in congress.
"Everyone has given their backing to the new Supreme Court," tweeted Freddy Guevara, a leader of the opposition-led congress.
